diff --git a/Lib/test/test_profiling/test_sampling_profiler/test_integration.py b/Lib/test/test_profiling/test_sampling_profiler/test_integration.py
index 3487647b76683e..385607c328bfc4 100644
--- a/Lib/test/test_profiling/test_sampling_profiler/test_integration.py
+++ b/Lib/test/test_profiling/test_sampling_profiler/test_integration.py
@@ -508,7 +508,10 @@ def test_sampling_with_collapsed_export(self):
                 if ";" in stack_trace:
                     stack_parts = stack_trace.split(";")
                     for part in stack_parts:
-                        # Each part should be file:function:line
+                        # Special frames, such as <GC> and unreadable frames,
+                        # do not have file and line information.
+                        if part.startswith("<") and part.endswith(">"):
+                            continue
                         self.assertIn(":", part)
 
     def test_sampling_all_threads(self):
diff --git a/Misc/NEWS.d/next/Tests/2026-11-07-01-45-00.gh-issue-160334.F2n8qA.rst b/Misc/NEWS.d/next/Tests/2026-11-07-01-45-00.gh-issue-160334.F2n8qA.rst
new file mode 100644
index 00000000000000..458f192ce0be77
--- /dev/null
+++ b/Misc/NEWS.d/next/Tests/2026-11-07-01-45-00.gh-issue-160334.F2n8qA.rst
@@ -0,0 +1,2 @@
+Fix the sampling profiler integration test for special frames without file and
+line information.